San Martín, J., Espinosa, A., Zanetti, S., Hauenstein, E., Ojeda, N., Arriagada. C. 2008. Composición y estructura de la vegetación epífita vascular en un bosque primario de Olivillo (Aextoxicon punctatum R. et P.) en el sur de Chile.  Ecología Austral. 18:1-11. aes%20et%20al.pdf

Aranda, E., Sampedro, I., Díaz, R., García-Sanchez, M., Arriagada, C., Ocampo, J.A., and García-Romera. I. 2008. Transformación de los residuos procedentes del olivo mediante cepas fúngicas. En: Tópicos sobre diversidad, ecología y usos de los hongos microscópicos en Iberoamérica.Heredia, G (Editora) (CYTED) e Instituto de Ecologia, A.C. Xalapa, Veracruz. México.386 pp. Chapter 15: 121-167. ISBN:970-709-104-5.

Arriagada, C. 2008. Alternativas de reutilización de residuos y fitoremediación mediante la interacción entre microorganismos promotores del crecimiento vegetal y especies forestales de interés comercial (Eucalyptus globulus y Quillaja saponaria). En: Nuevos paradigmas en la gestión forestal sostenible. (Herrera, M; Rodríguez, F; Molina, J; Moreno, N. Editores). Capítulo 16: 126-134 p.

ISBN: 84-691-9465-2.

Genome Sequence of Brevundimonas sp., an Arsenic Resistant Soil Bacterium

Author Full Names: Soto, Javiera; Charles, Trevor C.; Lynch, Michael D. J.; Larama, Giovanni; Herrera, Hector; Arriagada, Cesar


Brevundimonas sp. is a bacteria able to grow in metal(loid) contaminated soil from Puchuncavi Valley, central Chile. This study has isolated a bacterial strain capable of growth under high doses of arsenic (As) (6000 mg L-1), and a draft genome sequence was generated. Additionally, real-time PCR was performed to examine the effect of As on some genes related to As resistance. Results demonstrated a total of 3275 predicted annotated genes with several genes related to the ars operon, metal(loid) resistance-related genes, metal efflux pumps, and detoxifying enzymes. Real-time PCR showed that the arsB involved in the efflux of As was down-regulated, whereas arsR, arsH, and ACR3 did not show differences with the addition of As. Our study provides novel evidence of diverse As regulating systems in tolerant bacteria that will lead to a better understanding of how microorganisms overcome toxic elements and colonize As contaminated soils and to the possible use of their specific properties in bioremediation.

DOI: 10.3390/d13080344

UT (Unique WOS ID): WOS:000688989600001

Cell Wall Calcium and Hemicellulose Have a Role in the Fruit Firmness during Storage of Blueberry (Vaccinium spp.)

Author Full Names: Olmedo, Patricio; Zepeda, Baltasar; Rojas, Barbara; Silva-Sanzana, Christian; Delgado-Rioseco, Joaquin; Fernandez, Kamila; Balic, Ivan; Arriagada, Cesar; Moreno, Adrian A.; Defilippi, Bruno G.; Campos-Vargas, Reinaldo

Source Title: PLANTS-BASEL

The firmness of blueberry is one of its most significant quality attributes. Modifications in the composition of the cell wall have been associated with changes in the fruit firmness. In this work, cell wall components and calcium concentration in two blueberry cultivars with contrasting firmness phenotypes were evaluated at harvest and 30 days cold storage (0 degrees C). High performance anion-exchange chromatography with pulse amperometric detector (HPAEC-PAD) analysis was performed using the Emerald (firmer) and Jewel (softer) blueberry cultivars, showing increased glucose in the firmer cultivar after cold storage. Moreover, the LM15 antibody, which recognizes xyloglucan domains, displayed an increased signal in the Emerald cultivar after 30 d cold storage. Additionally, the antibody 2F4, recognizing a homogalacturonan calcium-binding domain, showed a greater signal in the firmer Emerald blueberries, which correlates with a higher calcium concentration in the cell wall. These findings suggest that xyloglucan metabolism and a higher concentration of cell wall calcium influenced the firmness of the blueberry fruit. These results open new perspectives regarding the role of cell wall components as xyloglucans and calcium in blueberry firmness.


UT (Unique WOS ID): WOS:000634059500001

Effect of arbuscular mycorrhizal fungi and mycoremediated dry olive residue in lead uptake in wheat plants

Author Full Names: Garcia-Sanchez, Mercedes; Andrea Silva-Castro, Gloria; Sanchez, Alvaro; Arriagada, Cesar; Garcia-Romera, Inmaculada


This study aims to evaluate the role of the extraradical mycelium of arbuscular mycorrhizal fungi in Pb transport. We also investigate how these fungi, alone or combined with the mycoremediated dry olive residue (MDOR), provide protection against Pb. We established a container system consisting of a central compartment and two lateral compartments separated by a hydrophobic membrane. The central compartment was filled with sterilized soil in which wheat plants, inoculated and non-inoculated with Funneliformis mosseae, were grown. The lateral compartments were filled with sterile, Pb-contaminated or MDOR-amended soil or combinations of both. In contrast to shoots and grains, wheat roots accumulated larger amounts of Pb with or without applications of MDOR. The extraradical mycelium (ERM) and the glomalin related protein content were significantly boosted by adding MDOR to Pb-contaminated soil samples. Wheat mot biomass was decreased as the result of Pb contamination with no increases in plant phosphorous (P) uptake. However, MDOR, when added to Pbcontaminated soil samples, only boosted the accumulation of P in roots, with P content and biomass remaining unchanged in wheat shoots and grains. Our study highlights the role of the ERM in Pb transport its accumulation in wheat mots and how the protection effect exerted by AMF seemed to rely on MDOR application by increasing the P uptake rather than Pb.

DOI: 10.1016/j.apsoil.2020.103838

UT (Unique WOS ID): WOS:000609456600020