Publicaciones
2009
Arriagada, C., Aranda, E., Sampedro, I., García-Romera, I. and Ocampo. JA. 2009. Contribution of the saprobic fungi Trametes versicolor and Trichoderma harzianum and the arbuscular mycorrhizal fungi Glomus deserticola and G. claroideum to arsenic tolerance of Eucalyptus globulus. Bioresource Technology. 100:6250–6257. (WoS. Impact Factor:4.453). https://doi.org/10.1016/ j.biortech.2009.07.010
Arriagada, C., Aranda, E., Sampedro, I., García-Romera, I. and Ocampo. JA. 2009. Differential effect of Trametes versicolor and Coriolopsis rigida on the action of the arbuscular mycorrhizal fungus Glomus deserticola on the copper tolerance of Eucalyptus globulus. Chemosphere. 77:273–278.(WoS. Impact Factor: 3.054). https://doi.org/10.1016/ j.chemosphere.2009.07.042
Arriagada, C., Sampedro, I., García-Romera, I. and Ocampo. JA. 2009. Improvement of growth of Eucalyptus globulus and soil biological parameters by amendment with sewage sludge and inoculation with arbuscular mycorrhizal and saprobe fungi. Science of the Total Environment. 407:4799–4806. (WoS. Impact Factor:2.579). https://doi.org/10.1016/ j.scitotenv.2009.05.021
Arriagada, C., Pacheco, P., Pereira, G., Machuca, A., Alvear, M. and J A Ocampo. 2009. Effect of arbuscular mycorrhizal fungal inoculation on Eucalyptus globulus Labill. seedlings and some soil enzyme activities under application of sewage sludge amendment. Journal of Soil Science and Plant Nutrition. 9(2): 89-101. (WoS. Impact Factor: 0.596). http://dx.doi.org/10.4067/S0718-27912009000200001
Aranda, E., Sampedro, I., Díaz, R., García-Sanchez, M., Arriagada, C., Ocampo, J.A., and García-Romera. I. 2009. The effects of the arbuscular mycorrhizal fungus Glomus deserticola on growth of tomato plants grown in the presence of olive mill residues modified by treatment with saprophytic fungi. Symbiosis. 47, 133–140. (ISI. Impact Factor:0.611). https://link.springer.com/content/pdf/ 10.1007%2FBF03179973.pdf
Laboratorio de Biorremediación
Departamento de Cs. Forestales
Universidad de La Frontera
Avenida Francisco Salazar 01145
Temuco, Chile
Casilla 54-D
Author Full Names: Arriagada, C.; Aranda, E.; Sampedro, I.; Garcia-Romera, I.; Ocampo, J. A.
Source Title: BIORESOURCE TECHNOLOGY
The presence of high concentrations of arsenic (As) decreased the shoot and root dry weight, chlorophyll and P and Mg content of Eucalyptus globulus colonized with the arbuscular mycorrhizal (AM) fungi Glomus deserticola or G. claroideum, but these parameters were higher than in non-AM plants. As increased the percentage of AM length colonization and succinate dehydrogenase (SDH) activity in the root of E. globulus. Trichoderma harzianum, but not Trametes versicolor, increased the shoot and root dry weight, chlorophyll content, the percentage of AM root length colonization and SDH activity of E. globulus in presence of all As concentrations applied to soil when was inoculated together with G. claroideum. AM fungi increased shoot As and P concentration of E. globulus to higher level than the non-AM inoculated controls. The contribution of the AM and saprobe fungi to the translocation of As from root to shoot of E. globulus is discussed. (C) 2009 Elsevier Ltd. All rights reserved.
DOI: 10.1016/j.biortech.2009.07.010
UT (Unique WOS ID): WOS:000270147700016
Author Full Names: Arriagada, C.; Aranda, E.; Sampedro, I.; Garcia-Romera, I.; Ocampo, J. A.
Source Title: CHEMOSPHERE
The presence of high levels of Cu in soil decreases the shoot and root dry weights of Eucalyptus globulus. However, higher plant tolerance of Cu has been observed in the presence of the arbuscular mycorrhizal (AM) fungus Glomus deserticola. The hyphal length of G. deserticola was sensitive to low Cu concentrations, and the percentage of AM root colonisation and the metabolic activity of the AM fungus were also decreased by Cu. Therefore, a direct effect of Cu on the development of the AM fungus inside and outside the root cannot be ruled out. E. globulus colonised by G. deserticola had higher metal concentrations in the roots and shoots than do non-mycorrhizal plants; however, the absence of a higher root to shoot metal ratio in the mycorrhizal plants (1.70 +/- 0.11) indicated that G. deserticola did not play a filtering/sequestering role against Cu. The saprobe fungi Coriolopsis rigida and Trametes versicolor were able to remove Cu ions from the asparagine-glucose growth media. However, plants inoculated with C. rigida and T versicolor did not accumulate more Cu than non-inoculated controls, and the growth of the plant was not increased in the presence of these fungi. However, C. rigida increased the shoot dry weight. AM root length colonisation, and metabolic mycelial activity of plants colonised with G. deserticola in the presence of Cu: only this saprobe-AM fungus combination increased the tolerance of E. globulus to Cu. Inoculation with G. deserticola and C. rigida increased the E. globulus Cu uptake to levels reached by hyperaccumulative plants. (C) 2009 Elsevier Ltd. All rights reserved.
DOI: 10.1016/j.chemosphere.2009.07.042
UT (Unique WOS ID): WOS:000270609100019
Author Full Names: Arriagada, C.; Sampedro, I.; Garcia-Romera, I.; Ocampo, J.
Source Title: SCIENCE OF THE TOTAL ENVIRONMENT
Sewage Sludge is widely used as an organic soil amendment to improve soil fertility. We investigated the effects of sewage sludge (SS) application on certain biological parameters of Eucalyptus globulus Labill. The plant was either uninoculated OF inoculated with saprobe fungi (Coriolopsis rigida and Trichoderma harzianum) or arbuscular mycorrhizal (AM) fungi (Glomus deserticola and Gigaspora rosea). Sewage sludge was applied to the surface of experimental plots at rates of 0, 2,4, 6 and 8 g 100 g(-1) of soil. Inoculation with both AM and saprobe fungi in the presence of SS was essential for the promotion of plant growth. The AM, saprobe fungi and SS significantly increased dry shoot weight. The AM fungi induced a significant increase in Fluorescein diacetate (FDA) activity but did not increase beta-glucosidase activity. Addition of SS to AM-inoculated soil did not affect either FDA or alpha-glucosidase activities in plants from soil that was either uninoculated OF inoculated with the saprobe fungi. SS increased beta-glucosidase activity when it was applied at 4 g 100 g(-1). SS negatively affected AM colonization as well as the mycelium SDH activity for both mycorrhizal fungi. SS increased Eucalyptus shoot biomass and enhanced its nutrient status. Inoculation of the soil with C. deserticola Stimulated significant E. globulus growth and increases in shoot tissue content of N, P, K, Ca, Mg and Fe. Dual inoculation with G. deserticola and either of the saprobe fungi had positive effects on K, Ca, Mg and Fe contents. The application of 8 g 100 g(-1) of SS had no positive effects on plant nutrition. The experimental setup provided a suitable tool for evaluating SS in combination with saprobe and AM fungi as a biological fertiliser for its beneficial effects on E. globulus plant growth. (C) 2009 Elsevier B.V. All rights reserved.
DOI: 10.1016/j.scitotenv.2009.05.021
UT (Unique WOS ID): WOS:000268371500002
Author Full Names: Arriagada, Cesar; Pacheco, Patricio; Pereira, Guillermo; Machuca, Angela; Alvear, Marysol; Antonio Ocampo, Juan
Source Title: REVISTA DE LA CIENCIA DEL SUELO Y NUTRICION VEGETAL
The sewage sludge (SS) represents a source of organic matter although its increasing accumulation need a suitable environmental treatment. Our study analyzed the effect of the interaction between sewage sludge (doses 0, 2, 4, 6 and 8 g per 100 g of soil) and Eucalyptus globulus nursery seedlings inoculated with arbuscular mycorrhizal (AM) fungi as a fertilizers alternative and evaluate its effect on some soil enzyme activities. The seedlings were inoculated with Glomus claroideum, Glomus viscosum, Glomus intraradices and Glomus constrictum, establishing a uninoculated control. The sewage sludge application increased the shoot dry weight and the inoculation with G. viscosum was more effective at 6 g of SS in 100 g soil. The root colonization and succinate dehydrogenase activity was decreased by all doses of SS used. The Fluorescein diacetate activity was increased by the AM inoculation but not by sewage sludge. The beta-glucosidase activity was increased in presence of SS at 6 g 100 g(-1). The N and P uptake by the E. globulus plants was higher in presence of sewage sludge and more effective with G. viscosum plant inoculation. In conclusion the application of these amounts of SS promoted the plant growth and can be regarded as a successful biotechnological tool for the greenhouse plant production.
DOI: -
UT (Unique WOS ID): WOS:000269531800001
Author Full Names: Aranda, E.; Sampedro, I.; Diaz, R.; Garcia-Sanchez, M.; Arriagada, C. A.; Ocampo, J. A.; Garcia-Romera, I.
Source Title: SYMBIOSIS
Olive oil extraction generates large amounts of olive mill residues (DOR) which may be used as fertilizer. The influence of arbuscular mycorrhizal (AM) oil the phytotoxicity of dry olive residue (DOR) transformed with saprophytic fungi studied. Aqueous extraction of DOR gave an (ADOR) fraction and all exhausted (SDOR) fraction. both of which had less phytotoxicity for tomato than the original DOR. The saprophytic fungi Trametes versicolor and Pycnoporus cinnabarinus further decreased the phytotoxicity of ADOR and SDOR on tomato. The decrease of phenols concentration and the differences in the level of laccase activity caused by these fungi suggest did not account fully for the reduced phytoxicity but the fact that the higher hydrolytic enzyme activity of P. cinnabarinus. paralleled the decrease of phytotoxicity. indicates that these enzymes seem to be involved. The AM fungus Glomus deserticola increased or exacerbated the beneficial effect of SDOR incubated with saprophytic fungi. in terms of dry weight of tomato plants. The percentage of root length colonized by G. deserticola strongly decreased in presence of DOR, but the level of mycorrhization was higher in presence of ADOR or SDOR. Our results suggest that the combination of aqueous extraction and incubation with saprophytic fungi will open the way for the use of olive oil extraction residues as organic amendment in agricultural soils.
DOI: 10.1007/BF03179973
UT (Unique WOS ID): WOS:000266468000002