P Olmedo, G Núñez-Lillo, J Vidal, Cl Leiva, B Rojas,
K Sagredo, César Arriagada, B G. Defilippi, A G. Pérez-Donoso,
Claudio Meneses, S Carpentier, R Pedreschi, Reinaldo Campos-Vargas. Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries. Food Chemistry 2023. 135498. (WOS. Impact Factor 9.231 Q1). 

T Sanhueza, H Herrera, César Arriagada. Contribution of Leaf‐Associated Microorganisms from Native Andean Ericaceae against Botrytis cinerea in Vaccinium corymbosum Cultivars. 2023.  Journal of Soil Science and Plant Nutrition.  (WOS. Impact Factor 3.600 Q1).


Alejandra Fuentes-Quiroz, Hector Herrera, Javier Ortiz, Cesar Arriagada, Emilio Jorquera-Fontena. 2023. Rhizosphere-inhabiting fungi isolated from native plants of the atacama desert affect leaf traits of ‘chardonnay’ grapevines (Vitis viniferaL.). Rhizosphere, Volume 27, 100715. (WOS. Impact Factor 3.437 Q1).




Proteomic and metabolomic integration reveals the effects of pre-flowering cytokinin applications on central carbon metabolism in table grape berries

Consumers around the world prefer high quality table grapes. To achieve higher quality traits at ripening, grapevine producers apply different plant growth regulators. The synthetic cytokinin forchlorfenuron N– (2–chloro–4–pyridinyl)–N′–phenylurea (CPPU) is widely used, its effect on grape quality is poorly understood. We hypothesized that the use of CPPU in pre-flowering can lead to changes in the metabolism that affects grape quality at harvest. Therefore, we investigated the role of CPPU applications on the quality of grapes by inte- grating proteomics and metabolomics. CPPU-treated grapevines showed a significant increase in berry size and firmness. Proteomic analyses indicated that CPPU-treated berries accumulated enzymes associated with carbo- hydrate metabolism, glycolysis, and tricarboxylic acid (TCA) cycle at harvest. Metabolomic analyses showed shifts in the abundance of compounds associated with carbohydrate metabolism and TCA cycle in CPPU-treated grapes. These findings suggest that CPPU applications modulate central carbon metabolism, improving grape berry quality.

Author Full Names:

P. Olmedo, G. Núñez-Lillo, J. Vidal, C. Leiva, B. Rojas,
K. Sagredo, César Arriagada, B. G. Defilippi, A. G. Perez-Donoso,
Claudio Meneses, Sebastien Carpentier, R. Pedreschi, Reinaldo Campos-Vargas

Source Title: Food Chemistry


Contribution of Leaf‐Associated Microorganisms from Native Andean Ericaceae against Botrytis cinerea in Vaccinium corymbosum Cultivars

In this study, the diversity of leaf-associated microorganisms of the native Andean ericaceous plants Gaultheria pumila and Empetrum rubrum was screened to identify and characterize microorganisms with plant growth promotion and biocontrol capability against the phytopathogenic fungus Botrytis cinerea affecting Vaccinium corymbosum cultivars. Microbial strains with biocontrol potential against Botrytis cinerea were selected, and in vivo tests were performed to evaluate the biocon- trol activity of the selected strains. The fungi Epicoccum nigrum (strains HFE11 and HFG20), Epicoccum layuense (strain HFG13), and Aspergillus sp. (strain HFG1), the yeasts Aureobasidium pullulans (strains BFG22 and BFG24) Sporobolomy- ces roseus (strains BFE10 and BFE11), and the bacteria Bacillus mycoides (strains BFE4 and BFE14), Bacillus sp. (strain BFG8), Pseudomonas fluorescens (strain BFE6), and Pseudomonas sp. (strain BFG21) were isolated. In vitro biocontrol activity of the selected strains (BFE14, BFE6, and HFG13) showed inhibition percentages ranging from 60 to 80%. Most of the isolates were able to produce Exopolysaccharides, Siderophore, Indole-3-acetic acid, P-solubilization and Ammonia to different levels. The in vivo experiments showed that the inoculation of the isolates BFG22, BFE6, and HFG13 on V. corymbosum leaves before infection avoids severe damage to the infected tissues. Additionally, BFG22 decreases the lipid peroxidation levels (malondialdehyde 36% lower) when the leaves were infected with B. cinerea. Our results provide evi- dence of beneficial traits of microorganisms inhabiting the phyllosphere of native Ericaceae which can be used as microbial inoculants in agricultural production. These beneficial effects enhance plant growth and avoid damage by B. cinerea in V. corymbosum cultivars.

Author Full Names:

T Sanhueza · H Herrera · César Arriagada

Source Title:

Journal of Soil Science and Plant Nutrition


Inoculation of Triticum Aestivum L. (Poaceae) with Plant-Growth-Promoting Fungi Alleviates Plant Oxidative Stress and Enhances Phenanthrene Dissipation in Soil

Author Full Names: Lagos, Claudio; Larsen, John; Fuentes, Alejandra; Herrera, Hector; Garcia-Romera, Inmaculada; Campos-Vargas, Reinaldo; Arriagada, Cesar


Polycyclic aromatic hydrocarbons (PAHs) are strong toxic compounds mainly released to the environment during combustion of fossil fuels, and have strong toxic effects on living organisms, with soil being one of their main reservoirs. High PAH levels in soils can interfere with plant growth and biomass production, causing several losses of diversity. In this study, we evaluated the effects of the co-inoculation of Trichoderma viride and Funneliformis mosseae on PAH dissipation and alleviation of oxidative stress in Triticum aestivum L. (wheat) plants growing in a phenanthrene-spiked soil. We determined the effect of single and dual fungal inoculation on phenanthrene dissipation rates, soil enzyme activities, dry biomass, antioxidant enzymes, lipid peroxidation, and organic acid exudation of plants growing in a soil spiked with phenanthrene at 500 and 1000 mg kg(-1) soil. The coinoculation with T. viride and F. mosseae resulted in a high phenanthrene dissipation from the soil. Also, dry biomass, soil enzymes, antioxidant response, organic acid exudation and phenanthrene content in roots were increased by the dual inoculation treatments, whereas lipid peroxidation and phenanthrene content in shoots were reduced. Our results show that the co-inoculation with these two soil fungi significantly promotes phenanthrene dissipation from soil and contributes to alleviating oxidative damage in wheat plants exposed to high levels of phenanthrene.

DOI: 10.3390/agronomy11030411

UT (Unique WOS ID): WOS:000633185500001

Effect of arbuscular mycorrhizal fungi and mycoremediated dry olive residue in lead uptake in wheat plants

Author Full Names: Garcia-Sanchez, Mercedes; Andrea Silva-Castro, Gloria; Sanchez, Alvaro; Arriagada, Cesar; Garcia-Romera, Inmaculada


This study aims to evaluate the role of the extraradical mycelium of arbuscular mycorrhizal fungi in Pb transport. We also investigate how these fungi, alone or combined with the mycoremediated dry olive residue (MDOR), provide protection against Pb. We established a container system consisting of a central compartment and two lateral compartments separated by a hydrophobic membrane. The central compartment was filled with sterilized soil in which wheat plants, inoculated and non-inoculated with Funneliformis mosseae, were grown. The lateral compartments were filled with sterile, Pb-contaminated or MDOR-amended soil or combinations of both. In contrast to shoots and grains, wheat roots accumulated larger amounts of Pb with or without applications of MDOR. The extraradical mycelium (ERM) and the glomalin related protein content were significantly boosted by adding MDOR to Pb-contaminated soil samples. Wheat mot biomass was decreased as the result of Pb contamination with no increases in plant phosphorous (P) uptake. However, MDOR, when added to Pbcontaminated soil samples, only boosted the accumulation of P in roots, with P content and biomass remaining unchanged in wheat shoots and grains. Our study highlights the role of the ERM in Pb transport its accumulation in wheat mots and how the protection effect exerted by AMF seemed to rely on MDOR application by increasing the P uptake rather than Pb.

DOI: 10.1016/j.apsoil.2020.103838

UT (Unique WOS ID): WOS:000609456600020

Controlled mycorrhization of the endemic Chilean orchidChloraea gavilu(Orchidaceae)

Author Full Names: Pereira, Guillermo; Herrera, Hector; Arriagada, Cesar; Cid, Hertes; Garcia, Jose Leonardo; Atala, Cristian


Orchids require mycorrhizal fungi (OMF) for their germination and growth. Propagation and re-introduction initiatives would likely require inoculation with such fungi. All Chilean Orchidaceae species are terrestrial and likely associate with OMF. We collected adult individuals of the endemic Chilean orchidChloraea gaviluand transported them to a glasshouse where we obtained mature capsules through manual auto-pollination. We asymbiotically germinated seeds in vitro using Malmgren Modified Terrestrial Orchid-Medium (MM). Embryos were put in glass flasks with MM where roots and leaves developed for 16 weeks. Plants were then transplanted to 165 mL pots and randomly separated into three groups; plants inoculated separately withCeratobasidiumOMFs isolated from two Chilean orchid species (Chloraea virescensandCodonorchis lessonii), and uninoculated (control) plants. Plants were then put in a growth chamber. Three months later, inoculated individuals showed pelotons inside parenchyma cells in the roots. Four months after inoculation, mycorrhizal plants had higher root and shoot biomass compared to control plants. At the end of the experiment, the highest mycorrhization was achieved with theCeratobasidiumstrain isolated fromC. lessonii. The artificial mycorrhization of endemic orchids could be a key strategy for future conservation and propagation initiatives, especially for endangered or commercially interesting species.

DOI: 10.1080/11263504.2020.1801875

UT (Unique WOS ID): WOS:000562194000001

Cell Wall Calcium and Hemicellulose Have a Role in the Fruit Firmness during Storage of Blueberry (Vaccinium spp.)

Author Full Names: Olmedo, Patricio; Zepeda, Baltasar; Rojas, Barbara; Silva-Sanzana, Christian; Delgado-Rioseco, Joaquin; Fernandez, Kamila; Balic, Ivan; Arriagada, Cesar; Moreno, Adrian A.; Defilippi, Bruno G.; Campos-Vargas, Reinaldo

Source Title: PLANTS-BASEL

The firmness of blueberry is one of its most significant quality attributes. Modifications in the composition of the cell wall have been associated with changes in the fruit firmness. In this work, cell wall components and calcium concentration in two blueberry cultivars with contrasting firmness phenotypes were evaluated at harvest and 30 days cold storage (0 degrees C). High performance anion-exchange chromatography with pulse amperometric detector (HPAEC-PAD) analysis was performed using the Emerald (firmer) and Jewel (softer) blueberry cultivars, showing increased glucose in the firmer cultivar after cold storage. Moreover, the LM15 antibody, which recognizes xyloglucan domains, displayed an increased signal in the Emerald cultivar after 30 d cold storage. Additionally, the antibody 2F4, recognizing a homogalacturonan calcium-binding domain, showed a greater signal in the firmer Emerald blueberries, which correlates with a higher calcium concentration in the cell wall. These findings suggest that xyloglucan metabolism and a higher concentration of cell wall calcium influenced the firmness of the blueberry fruit. These results open new perspectives regarding the role of cell wall components as xyloglucans and calcium in blueberry firmness.


UT (Unique WOS ID): WOS:000634059500001